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Turner Designs News - Vol. 25 Sept. 2012
Reliable Instruments for an Unreliable World

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Introduction
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George Turner probably didn’t envision fluorometers going to extreme depths or traveling great distances over extended periods of time when he started Turner Designs more than 40 years ago.  Particularly with our Cyclops-6K and C3 Submersible Fluorometers we’re providing instruments which enable researchers to study areas of the ocean that have minimally been studied in the past. We’re very excited to be part of our customers’ diverse explorations, which is probably why the longest section of our newsletter this month is Instruments in Action.

Thanks for taking us with you!

Best Regards,
Pam Mayerfeld

VP of Marketing & Sales
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NEW PRODUCTS IN THE SPOTLIGHT

In Situ
Detection of Tryptophan Using Turner Designs' Submersible Fluorometers
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Tryptophan is an amino acid dissolved in water that has a specific excitation and emission. It is classified as protein-like organic matter and sources may include water systems with high biological activity and wastewater or industrial discharge. Tryptophan is yet another parameter researchers can measure to track wastewater effluent, which may greatly impact habitats and wildlife.

Many of the instruments available for measuring fluorescence of Tryptophan are cumbersome, complex, expensive, and require a high degree of training and expertise to operate. These instruments may also provide too much information to end users who are looking for a simple measure of fluorescence response from, and relative changes of, Tryptophan in water.       
>>read more
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Cyclops-6K now available for Refined Fuels, Blue Green Algae, and Turbidity
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We’re excited to introduce new optical configurations for the Cyclops-6K Submersible Fluorometer. New applications include Refined Fuels, Phycocyanin, Phycoerythrin, and Turbidity in addition to previously announced Chlorophyll, CDOM/FDOM, and Crude Oil. The Cyclops-6K’s reinforced Titanium housing and specialized optical head allow it to reach depths of 6000 meters. Offering the same low power requirements, high performance, and long-term stability as the Cyclops-7 Submersible Fluorometer, the Cyclops-6K is designed with system integration in mind. The analog output is easily integrated into CTDs, ROVs, or AUVs to monitor the deepest parts of the ocean.

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NEW ACCESSORIES

New Submersible Logger for Cyclops-7 Sensors
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fluorometer A new submersible logger which integrates with any of Turner Designs' Cyclops-7 Fluorometers/Turbidimeters has been developed by Precision Measurement Engineering Inc. The logger can be configured with either stainless or titanium Turner Designs Cyclops-7-NC (no connector) sensors. The logger will record time, date, internal temperature, fluorescence or turbidity, and gain. Data are recorded on a removable SD card and the unit is powered by two alkaline C cell batteries, potentially powering the Cyclops-7 for up to a year or 30,000 sample measurements before requiring replacement. The system comes complete with configuration and visualization software. Users can select a sample interval and configure the logger for extended or short-term deployments.

For pricing and availability of the logger, contact PME

PME Headquarters & Manufacturing
1487 Poinsettia Ave., Suite 129
Vista, CA 92081
(760) 727-0300
Email This e-mail address is being protected from spambots. You need JavaScript enabled to view it
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Antifouling Copper Tape now available for C3 Submersible Fluorometers
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Copper is highly effective for minimizing bio-fouling of substrates. Turner Designs now offers Antifouling Copper Tape pieces designed for optimal coverage of our C3 Submersible Fluorometer’s optical head to reduce bio-fouling on and around the sensors. The combination of the antifouling copper tape used together with our 3-brush mechanical wiper provides the most efficient reduction in bio-fouling. Even when used without the wiper the antifouling copper tape dramatically reduces bio-fouling on the optical head. (View Tech Note). Packages of 15 copper tape pieces (PN 2300-506) are included with every new C3 shipment. Additional 15-packs are available for purchase. Contact us to request pricing.


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UV Solid Secondary Standards now available
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We now have UV Solid Secondary Standards for our C3 Submersible Fluorometers and Cyclops series Submersible Sensors. Instrumentation configured for UV analyses typically rely on the use of liquid standards, such as Quinine Sulfate, for ensuring instrument integrity. However, transporting and preparing liquid standards can be tedious and sometimes hazardous. The UV Solid Secondary Standard is compact, lightweight, and safe to use as a convenient way to quickly check the stability of our Tryptophan, Crude Oil, Refined Fuels, Dissolved Organics (DOM), and Brightener/Whitener sensors. To ensure data integrity, the UV Solid Secondary Standard can be read before and after a deployment or sampling event. Establishing a correlation between a primary standard’s known concentration and the UV Solid Secondary Standard’s signal eliminates the ongoing need for costly and time-consuming liquid calibration standards.

Part Numbers:
2300-902 for C3 Submersible Fluorometer (requires Solid Standard Cap part number 2300-905, previously sold as 2300-900)
2100-904 for Cyclops-7 units with stainless steel or titanium housing
2100-905 for Cyclops-7 units with plastic housing
2160-901 for Cyclops-6K units

Contact us to request pricing.
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INSTRUMENTS IN ACTION

C3 Submersible Fluorometers traversing the ocean to Japan and Australia
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On November 17th, 2011, Liquid Robotics launched four Wave Gliders planning to travel the longest distance at sea ever completed by an unmanned marine vehicle. The robots successfully travelled together from San Francisco to Hawaii and then embarked on separate routes across the Pacific, one pair heading for Japan and the other pair to Australia. All four Wave Gliders are equipped with C3 Submersible Fluorometers transmitting data on Chlorophyll, Crude Oil, & Turbidity. As of September 21, the gliders have between 1565 & 4180 miles to go before they complete their journeys (one hasn't been heard from since early August). Particularly exciting was the chlorophyll signal detected by one glider in the middle of the Pacific Ocean on June 20 and confirmed by its partner following behind on June 26.   For more information, visit www.liquidr.com fluorometer
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Cyclops-7 integrated into LakeESP Monitoring Buoy
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The Leibniz-Institute of Freshwater Ecology and Inland Fisheries (IGB), the largest limnological research institute in Germany, recently installed a LakeESP (Environmental Sensing Platform) in Lake Stechlin for monitoring various aquatic and meteorological parameters. The LakeESP is a complete monitoring buoy for long-term water quality monitoring and management. Water data include temperature, dissolved oxygen, Chlorophyll, PAR and pressure. Meteorological data include wind speed and direction, humidity, air temperature, barometric pressure, liquid precipitation and net long and short wave radiation. Data are collected every minute and telemetered via GSM cell phone to IGB headquarters every hour.  >>read more fluorometer
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Monitoring platform protects Penobscot Indian Nation, Culture
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A water quality monitoring platform in the Penobscot River is helping the Penobscot Indian Nation protect and restore the heart of their culture.

The platform floats in Dolby Pond, an impoundment on the Penobscot River and one of the more than 110 sites on rivers, streams and lakes that the Penobscot Nation’s Water Resources Program has been monitoring since the early ‘90s.

In the past 15 years, blooms in the main stem of the Penobscot River have become more severe and the phytoplankton community has shifted from a green algal dominant system to a system primarily dominated by cyanobacteria. The platform project began after a particularly bad cyanobacteria bloom in 2007 led the state of Maine to impose a fine on an upstream paper mill for excessive phosphorous discharges. >>read more
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A personal watercraft-based system for coastal open mapping
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Learn how Bret Webb, University of South Alabama, is using the Turner Designs C3 Submersible Fluorometer with CDOM/FDOM, refined fuels and crude oil optics as part of a personal watercraft-based system for coastal open mapping. – Download article published in Journal of Ocean Technology
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Knysna Basin Project utilizes C6 Multi-Sensor Platform with Chlorophyll and Turbidity Sensors
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Rhodes University Field Station is an adjunct of the Department of Zoology and Entomology. The University Department has made considerable advances in estuarine and oceanic studies over many years that have appeared in international peer reviewed journals. It is sited in the coastal town of Knysna, 400 km west of the university in Grahamstown. >>read more
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A temperature compensation method for CDOM fluorescence sensors in freshwater
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The effect of temperature on CDOM fluorescence was investigated in dystrophic freshwaters of Wisconsin and in aqueous standards. Laboratory experiments with two commercial in situ fluorometers showed that CDOM fluorescence intensity decreased as ambient water temperature increased. A temperature compensation equation was derived to standardize CDOM fluorescence measurements to a specific reference temperature. >> read more
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TECH CORNER

What Affects the Relative Fluorescence of Fluorescein?
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Fluorescein is a tracer dye used in flow studies, environmental, industrial, and biotech applications. There are many variations of fluorescein dye and each compound will produce slightly shifted excitation and emission maxima. However, these small shifts shouldn’t greatly affect the signal detected by fluorometers that are configured with broad band excitation/emission filters, especially if the compound used in the study is the same compound used to calibrate the fluorometer.

Aside from the molecular structure of different fluorescein dyes, temperature is a parameter that affects the fluorescence response of fluorescein. Generally, as temperature increases, the fluorescence response of fluorescein decreases, and vice versa. Temperature correction coefficients are available for correcting this change in fluorescence:

     [Fluorescein Temperature Coefficient = -0.0036 per degree Celsius]

There could be as much as 10% percent error if temperature is uncorrected and the temperature swings are 30 degrees or greater, such as boiler or cooling water applications.    >>read more
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FAQ's Online
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Have you ever asked the Question - how do I power and collect data from the Cyclops? The Answer is, our Cyclops sensors can be powered using any 3-15 volt power source. Cyclops sensors are analog output fluorometers that out put voltage only; data can be collected via any datalogger that accepts a 0-5 volt analog signal. The analog sensors will output measurements as long as power is being supplied. We sell a DataBank Handheld Datalogger, compatible with our entire Cyclops line, for easy data collection in the field. The DataBank allows for continuous measurements at a maximum sample rate of 1 second.

More information like this is available on our website. Turner Designs’ Technical Support Team has been posting Frequently Asked Questions for our instruments, standards and applications. This is on-going in order to provide updated pertinent advice to customer questions and concerns. Please keep a lookout for updates and feel free to submit questions to our support team.
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Bulkhead Connector Maintenance
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This may not be something you think about every day or at all for that matter, but Neoprene needs LOVE. The bulkhead connectors for our C-Series instruments require maintenance and lubrication to ensure that they don’t degrade and become leak points allowing instruments to flood with water, causing extensive damage. Mating surfaces of the connectors need to be lubricated on a regular basis with 3M™ Silicone Lubricant Spray, Loctite 8021 spray or equivalent.

You should avoid using silicone grease. DO NOT USE WD-40 - it will destroy the connectors.

Other things to remember:
•   Avoid nicks and cuts around contacts as these are the sealing surfaces.
•   Neoprene can be seriously degraded if exposed to direct sunlight or high ozone levels for extended periods of time.
•   Do not pull on cable to disconnect.
•   Avoid sharp bends at cable entry to connector.

If you have any questions regarding maintenance of connectors, please contact Technical Support.
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New Video: Calibrating the AquaFluor Handheld Fluorometer with Liquid Primary Standards
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This short video instructs users on how to calibrate their AquaFluor using a standard with a known concentration for the purpose of making quantitative measurements and estimating actual concentrations of a specific fluorophore in solution.
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GENERAL ANNOUNCEMENTS


Travel Stipend Award
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Rebecca Bernard of Dauphin Island Sea Lab in Alabama was the first recipient of our Travel Stipend.   Rebecca gave a presentation at the Ecological Society of America meeting in August in Portland, Oregon titled The Biogeochemistry of Oyster Restoration. Rebecca used a TD-700 Laboratory Fluorometer (which was obsoleted when we introduced the Trilogy) to look at Chlorophyll levels as a proxy for microbial biomass. Congratulations to Rebecca and thanks for using Turner Designs instruments in your research, Rebecca. Click here to view Rebecca’s presentation.
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Donation Program Award
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Congratulations to Natasha Gownaris of Stony Brook University who was awarded an AquaFluor Handheld Fluorometer/Turbidimeter via our Instrument Donation Program. The next award will be announced in December. Applications are due by November 1, 2012. Click here to learn more about Natasha’s research.
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Upcoming Events
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Oceans 2012 MTS/IEEEwebsite
Hampton Roads, Virginia, USA
Virginia Beach Convention Center - Booth# 1628
October 14-19, 2012

NALMS International Symposiumwebsite
Madison, Wisconsin, USA
Booth# TBD
November 7-9, 2012

AGU 2012 Fall Meetingwebsite
San Francisco, California, USA
Moscone Center - Booth# 537
December 3-7, 2012
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Turner Designs Inc. · 845 W. Maude Avenue · Sunnyvale · CA · 94085 · USA
Toll Free 877.316.8049 · Tel 408.749.0994 · Fax 408.749.0998 · Web www.turnerdesigns.com
© Turner Designs Inc. 2012 All Rights Reserved
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