| Introduction: Note from the Environmental Marketing Manager
In the Spotlight: Coming Soon! C6 Multi-Sensor Platform
Submersible Active Fluorometer in Action: New PhytoFlash Performance Data
Tom's Corner: Rhodamine WT as a Secondary Standard
Trilogy Laboratory Fluorometer - New Phosphate and Silicate Absorbance Modules
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Turner Designs Databank: What is it? How does it work? Why participate?
|Welcome to the Fall edition of the Turner Designs Newsletter. We are excited to announce a new submersible product, and two new absorbance applications for the Trilogy Laboratory Fluorometer. This has been an extraordinary year for new products at Turner. We have:
All of our instruments can be built for specified custom optics because we understand that research needs are not the same throughout varying environments and applications.
In this edition we'd like to highlight our new C6 Multi-Sensor Platform designed to provide intelligent interface capabilities, flexible optical sensor integration and increased power for long-term deployments. The C6 Multi-Sensor Platform design was initiated after an overwhelmingly positive response on the Cyclops-7 analog fluorometer. With increasing optical applications and the need for more flexibility we feel the C6 Multi-Sensor Platform adds excellent features such as a mechanical bio-fouling wiper and increasing benefits including a robust battery for extended deployments. We are excited to launch the new C6 Multi-Sensor Platform before the New Year!
Coming Soon! C6 Multi-Sensor Platform
The PhytoFlash is a solid state (patent pending) submersible active fluorometer designed to detect minimum fluorescence (Fo), maximum fluorescence (Fm), and quantum efficiency (Fv/Fm or yield) of phytoplankton. These data can be determined during extended deployments, CTD profiles, or in the laboratory. The Turner Designs submersible battery allows well over 45 days of logging at 15-minute intervals.
Question: When measuring the fluorescence of Chlorophyll a, can Rhodamine WT be used as a secondary standard?
Answer: Yes. Rhodamine WT is a suitable secondary standard for fluorometers that are equipped with chlorophyll a optics. The solid secondary standards that are offered by Turner Designs (see Table 1) are highly recommended because they are easy to use and deliver a stable signal. In situations where it is not possible to use the solid standard, Rhodamine WT is an excellent alternative. For customers who infrequently work with Rhodamine, an adjustable solid standard can be set to simulate a known concentration of Rhodamine WT, eliminating the need to keep liquid Rhodamine WT on hand. Exposure to sunlight has very little effect on Rhodamine WT but free chlorine will rapidly destroy Rhodamine WT dye. The maximum excitation wavelength of rhodamine WT is 558 nm and the maximum emission wavelength is 583 nm (see Figure 1).
Rhodamine WT Dilutions and Solid Secondary Adjustment
Turner Designs offers an excellent resource page that covers the preparation of dye standards. Figure 2 illustrates the visual characteristics of Rhodamine WT.
Trilogy Laboratory Fluorometer - New Phosphate and Silicate Absorbance Modules!
Inorganic Nutrient Analysis Using Turner Designs Trilogy Absorbance Module
Phosphate Absorbance Module:
Figure 2 shows comparison data from split water samples analyzed using a Bran & Luebbe Auto Analyzer for low level determination of inorganic nutrients and a Turner Designs Trilogy with a Phosphate Absorbance Module.
Inorganic phosphate is typically limiting and one of the three necessary nutrients used for primary production by terrestrial and aquatic plants. Monitoring of phosphate levels in water can help identify possible sources for introduction to aquatic systems. Measuring phosphate in aquatic environments can be a very important tool in understanding the health of a system or its "water quality".
Silicate Absorbance Module:
Preliminary work on silicate determination using Turner Designs Trilogy Silicate Absorbance Module shows a strong linear relationship from 1 - 100 µM silicate, with r2 value of 0.99 (Fig 2). Low concentration results show that we can detect down to 0.1 µM silicate concentration (Fig 1).
Silicate performance testing is currently underway with Romberg Tiburon Labs. Those data will be posted to the Turner Designs Databank for customer viewing. The Silicate Absorbance Module will be released and available for purchase soon.
|Turner Designs Databank|
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