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Please note, our office will be closed on Monday, May 29th, in observance of Memorial Day. We will resume business on Tuesday, May 30th. Please feel free to send us messages during this time and we will respond upon our return.
What variables will effect the linearity of a sample?
Fluorescence intensity is typically directly proportional (linear) to concentration.  However, when a concentration is too high, light cannot pass through the sample to cause excitation; thus very high concentrations can have very low fluorescence.  This happens when the sample is “quenched”.  The fluorometer readings will decrease, even though the concentration is still increasing.  Diluting a sample 1:1 or some other convenient ratio will check linearity.  If it is linear, the reading will decrease in direct proportion to the dilution.