Products & Accessories
Applications & Customer Care
Search - Contactenhanced_search
Case Studies
Turner Blog
Products & Updates
General Information
Contacts
Content Categories
Content Sections
**Upcoming Holiday**
Please note our office will be closed on Monday, May 28th in observance of the Memorial Day holiday. We will re-open for regular business on Tuesday, May 29th. Please feel free to send us correspondence during this time and we will reply to you when we return

What variables will effect the linearity of a sample?
Fluorescence intensity is typically directly proportional (linear) to concentration.  However, when a concentration is too high, light cannot pass through the sample to cause excitation; thus very high concentrations can have very low fluorescence.  This happens when the sample is “quenched”.  The fluorometer readings will decrease, even though the concentration is still increasing.  Diluting a sample 1:1 or some other convenient ratio will check linearity.  If it is linear, the reading will decrease in direct proportion to the dilution.