I have heard that fluorescence is temperature sensitive. Can I correct my data to compensate for this?
Fluorescence is temperature sensitive. As the temperature of the sample increases, the fluorescence decreases. For greatest accuracy, record the sample temperature and correct the sensor output for changes in temperature.
The following are Temperature Coefficients for common fluorescent applications:
If you need to correct various samples to a standard temperature the equation is:
Fr = Fs [n (Ts-Tr)]
Fr = the calculated fluorescence reading at the reference temperature, Tr.
Fs = the observed fluorescence reading of the sample at the time of reading the sample temperature, Ts.
e = the base of natural log.
n = a temperature coefficient.
Ts = the sample temperature at time of reading Fs.
Tr = the reference temperature.
Note that usually when presented, n has a negative value, but here the equation is arranged in simpler form, with n being positive. All temperatures are in degrees centigrade. For small temperature differences, the values may be used directly. For a two-degree rise in temperature for rhodamine WT, the reading will drop 5.2% (2 X 0.026, expressed as a percentage).