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AlgaeWatch Online Fluorometer FAQ

What causes the AlgaeWatch to give negative readings?

There are two possible reasons that the AlgaeWatch is displaying a negative number.

1.    If the liquid that is being measured generates a response lower than original blank, you will read a negative number.  If this is the case, you may need to re-calibrate using a different blank solution.

2.    Another possible cause for negative readings might be a fouled flowcell.  The AlgaeWatch has an optical compensation that will correct for biofouling up to a certain point.  Without cleaning, biofilm can continue to grow and it will at some point become thick enough to interfere with fluorescent readings.  Clean the flowcell and recalibrate as directed in page 10 of the operation manual.  If there is heavy biofouling follow the additional cleaning instructions on page 16 of the operation manual.

What is the maximum impedance for the 4 to 20mA output for the AlgaeWatch?

The maximum impedance load the 4-20 mA output can handle is 1Kohm.

What is the concentration of Rhodamine WT used in the AlgaeWatch Calibration kit P/N 6500-900?

The AlgaeWatch calibration solution is a concentration of 400ppb Rhodamine WT (2-500mL bottles).  The kit also includes syringes and cleaning brushes.  For pricing and availability contact Turner Designs Accessories at 1-877-316-8049.


How do you set the background % value for the AlgaeWatch?

The AlgaeWatch operation manual gives an overview of how to determine the background % value based on a default calibration standard value of 500.  It lists the following formula for use in determining the background value.  It is valid ONLY if the calibration standard value is still set at 500.
Filtrate fluorescence (x) / 5 = Background Fluorescence %

Another way to calculate the background fluorescence is the following:

Filtrate fluorescence (x) / Standard Value x 100

To determine filtrate fluorescence, filter a water sample through a GF/F or membrane filter to remove all algal cells. Then inject the filtrate into the AlgaeWatch to get the filtrate’s fluorescence response. NOTE: The AlgaeWatch must be calibrated prior to this step.

How can I connect my AlgaeWatch to a computer when there are no serial ports available?

If you do not have a serial port available on your computer you can connect your AlgaeWatch to an available USB port.  USB to serial adapters are available at most major electronics vendors.  These adapters will come with driver software.  You will want to make sure that the driver is installed and operating properly before you test your system out.  To verify operation you can use a communication program such as hyperterminal to verify the connection.

What does the AlgaeWatch Flowcell Cleaning Brush P/N 6000-350 look like?

Please refer to the picture below:


What does the AlgaeWatch Basket Strainer P/N 119-0103 look like?

Please refer to the picture below:


What are the specifications for the AlgaeWatch?